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mitomycin c  (MedChemExpress)
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MedChemExpress mitomycin c
TriStim-E6/E7 mRNA induced T cell activation and proliferation in vitro . (A, B) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were used to stimulate mouse splenocytes. The expression of CD69 (A) and CD25 (B) in splenic T cells was assessed by flow cytometry analysis. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. (C, D) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were treated with <t>mitomycin</t> <t>C</t> before co-cultured with mouse splenocytes. The proliferation of CFSE-prestained mouse splenic T cells were assessed by flow cytometry. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. Data are presented as the mean ± standard deviation (n = 3). Statistical analysis was conducted using an unpaired, two-tailed Student’s t-test; **P ≤ 0.01, ***P ≤ 0.001.
Mitomycin C, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mitomycin c treated mouse embryonic fibroblasts mefs  (Thermo Fisher)
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Thermo Fisher mitomycin c treated mouse embryonic fibroblasts mefs
TriStim-E6/E7 mRNA induced T cell activation and proliferation in vitro . (A, B) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were used to stimulate mouse splenocytes. The expression of CD69 (A) and CD25 (B) in splenic T cells was assessed by flow cytometry analysis. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. (C, D) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were treated with <t>mitomycin</t> <t>C</t> before co-cultured with mouse splenocytes. The proliferation of CFSE-prestained mouse splenic T cells were assessed by flow cytometry. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. Data are presented as the mean ± standard deviation (n = 3). Statistical analysis was conducted using an unpaired, two-tailed Student’s t-test; **P ≤ 0.01, ***P ≤ 0.001.
Mitomycin C Treated Mouse Embryonic Fibroblasts Mefs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mitomycin c  (Thermo Fisher)
96
Thermo Fisher mitomycin c
TriStim-E6/E7 mRNA induced T cell activation and proliferation in vitro . (A, B) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were used to stimulate mouse splenocytes. The expression of CD69 (A) and CD25 (B) in splenic T cells was assessed by flow cytometry analysis. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. (C, D) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were treated with <t>mitomycin</t> <t>C</t> before co-cultured with mouse splenocytes. The proliferation of CFSE-prestained mouse splenic T cells were assessed by flow cytometry. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. Data are presented as the mean ± standard deviation (n = 3). Statistical analysis was conducted using an unpaired, two-tailed Student’s t-test; **P ≤ 0.01, ***P ≤ 0.001.
Mitomycin C, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mitomycin c mmc  (Fisher Bioreagents)
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Fisher Bioreagents mitomycin c mmc
TriStim-E6/E7 mRNA induced T cell activation and proliferation in vitro . (A, B) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were used to stimulate mouse splenocytes. The expression of CD69 (A) and CD25 (B) in splenic T cells was assessed by flow cytometry analysis. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. (C, D) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were treated with <t>mitomycin</t> <t>C</t> before co-cultured with mouse splenocytes. The proliferation of CFSE-prestained mouse splenic T cells were assessed by flow cytometry. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. Data are presented as the mean ± standard deviation (n = 3). Statistical analysis was conducted using an unpaired, two-tailed Student’s t-test; **P ≤ 0.01, ***P ≤ 0.001.
Mitomycin C Mmc, supplied by Fisher Bioreagents, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mitomycin c  (Kirin Co Ltd)
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Kirin Co Ltd mitomycin c
TriStim-E6/E7 mRNA induced T cell activation and proliferation in vitro . (A, B) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were used to stimulate mouse splenocytes. The expression of CD69 (A) and CD25 (B) in splenic T cells was assessed by flow cytometry analysis. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. (C, D) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were treated with <t>mitomycin</t> <t>C</t> before co-cultured with mouse splenocytes. The proliferation of CFSE-prestained mouse splenic T cells were assessed by flow cytometry. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. Data are presented as the mean ± standard deviation (n = 3). Statistical analysis was conducted using an unpaired, two-tailed Student’s t-test; **P ≤ 0.01, ***P ≤ 0.001.
Mitomycin C, supplied by Kirin Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mitomycin c  (Selleck Chemicals)
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Selleck Chemicals mitomycin c
TriStim-E6/E7 mRNA induced T cell activation and proliferation in vitro . (A, B) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were used to stimulate mouse splenocytes. The expression of CD69 (A) and CD25 (B) in splenic T cells was assessed by flow cytometry analysis. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. (C, D) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were treated with <t>mitomycin</t> <t>C</t> before co-cultured with mouse splenocytes. The proliferation of CFSE-prestained mouse splenic T cells were assessed by flow cytometry. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. Data are presented as the mean ± standard deviation (n = 3). Statistical analysis was conducted using an unpaired, two-tailed Student’s t-test; **P ≤ 0.01, ***P ≤ 0.001.
Mitomycin C, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mitomycin c  (Fisher Scientific)
86
Fisher Scientific mitomycin c
TriStim-E6/E7 mRNA induced T cell activation and proliferation in vitro . (A, B) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were used to stimulate mouse splenocytes. The expression of CD69 (A) and CD25 (B) in splenic T cells was assessed by flow cytometry analysis. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. (C, D) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were treated with <t>mitomycin</t> <t>C</t> before co-cultured with mouse splenocytes. The proliferation of CFSE-prestained mouse splenic T cells were assessed by flow cytometry. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. Data are presented as the mean ± standard deviation (n = 3). Statistical analysis was conducted using an unpaired, two-tailed Student’s t-test; **P ≤ 0.01, ***P ≤ 0.001.
Mitomycin C, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mitomycin c  (Sanofi)
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Sanofi mitomycin c
TriStim-E6/E7 mRNA induced T cell activation and proliferation in vitro . (A, B) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were used to stimulate mouse splenocytes. The expression of CD69 (A) and CD25 (B) in splenic T cells was assessed by flow cytometry analysis. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. (C, D) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were treated with <t>mitomycin</t> <t>C</t> before co-cultured with mouse splenocytes. The proliferation of CFSE-prestained mouse splenic T cells were assessed by flow cytometry. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. Data are presented as the mean ± standard deviation (n = 3). Statistical analysis was conducted using an unpaired, two-tailed Student’s t-test; **P ≤ 0.01, ***P ≤ 0.001.
Mitomycin C, supplied by Sanofi, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TriStim-E6/E7 mRNA induced T cell activation and proliferation in vitro . (A, B) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were used to stimulate mouse splenocytes. The expression of CD69 (A) and CD25 (B) in splenic T cells was assessed by flow cytometry analysis. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. (C, D) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were treated with mitomycin C before co-cultured with mouse splenocytes. The proliferation of CFSE-prestained mouse splenic T cells were assessed by flow cytometry. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. Data are presented as the mean ± standard deviation (n = 3). Statistical analysis was conducted using an unpaired, two-tailed Student’s t-test; **P ≤ 0.01, ***P ≤ 0.001.

Journal: Frontiers in Immunology

Article Title: A novel mRNA-based therapeutic vaccine elicits robust anti-tumor immunity against HPV-associated malignancies

doi: 10.3389/fimmu.2026.1823374

Figure Lengend Snippet: TriStim-E6/E7 mRNA induced T cell activation and proliferation in vitro . (A, B) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were used to stimulate mouse splenocytes. The expression of CD69 (A) and CD25 (B) in splenic T cells was assessed by flow cytometry analysis. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. (C, D) HEK293 cells co-transfected with 0.25 μg of mb-α-CD3 mRNA and 5 μg of TriStim-E6/E7 mRNA, were treated with mitomycin C before co-cultured with mouse splenocytes. The proliferation of CFSE-prestained mouse splenic T cells were assessed by flow cytometry. Control groups included HEK293 cells transfected with empty LNPs, 0.25 μg of mb-α-CD3 mRNA alone, or combination of 0.25 μg of mb-α-CD3 mRNA and 5 μg of E6/E7-P2P16-MITD mRNA. Data are presented as the mean ± standard deviation (n = 3). Statistical analysis was conducted using an unpaired, two-tailed Student’s t-test; **P ≤ 0.01, ***P ≤ 0.001.

Article Snippet: To assess T cell proliferation, mRNA-transfected HEK293 cells were treated with 15 μg/mL mitomycin C (Cat. #HY-13316, MedChemExpress, Shanghai, China) for 4 hours to inhibit cell growth.

Techniques: Activation Assay, In Vitro, Transfection, Expressing, Flow Cytometry, Control, Cell Culture, Standard Deviation, Two Tailed Test